Queens manager Kenny Brannigan is hoping that Allan Johnston and Daniel Carmichael pass lunchtime fitness tests for the trip to Fife.
If they are ruled out, he will have only eight first-team squad members, with Rocco Quinn and Paul Burns injured and Steven McKenna banned.
Cowdenbeath manager Jimmy Nicholl: "It is always disappointing to be beaten. However, we put in a good performance against Falkirk, especially in defence.
"We have something to build on now, as everyone will take confidence from that game.
"We will look to improve our record against Queen of the South that has seen us ship six goals to them in two games so far this season."
Queen of the South manager Kenny Brannigan: "It is getting beyond the bare bones here and no disrespect to the youth players but they are not ready for first-team football.
"We could have two untested kids in our starting 11 and four on the bench, so it will be a tough shift at Central Park.
"We want to mount a serious challenge for promotion but it is getting harder due to our squad."
Source: BBC Sport
Source: BBC Sport